The total carotenoid content was calculated using the following formula: Carotenoids content μg / g = A × V mL × 1 0 4 A 1 cm 1 % × P g where A = Absorbance; V = Total extract volume; P = sample weight; A1cm1% = 2592 (β-carotene Extinction Coefficient in petroleum ether).
What are total carotenoids?
Carotenoids are a class of more than 750 naturally occurring pigments synthesized by plants, algae, and photosynthetic bacteria (1). These richly colored molecules are the sources of the yellow, orange, and red colors of many plants. Fruit and vegetables provide most of the 40 to 50 carotenoids found in the human diet.
What is the value of carotenoids?
Carotenoids are beneficial antioxidants that can protect you from disease and enhance your immune system. Provitamin A carotenoids can be converted into vitamin A, which is essential for growth, immune system function, and eye health.
How do you test for carotenoids?
A variety of methods have been employed to detect the carotenoids in food samples ranging from thin layer chromatography, to high pressure liquid chromatography (HPLC) and combination of HPLC with mass spectrometry including MALDI-TOF.
How is beta-carotene measured?
The beta-carotene test measures the level of beta-carotene in the blood. Blood is drawn from a vein (venipuncture), usually from the inside of the elbow or the back of the hand. A needle is inserted into the vein, and the blood is collected in an air-tight vial or a syringe.
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